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SSR markers to detect gene flow from upland to mocó cotton Rev. Ciênc. Agron.
Dantas,Ana Carolina Assis; Barroso,Paulo Augusto Vianna; Hoffmann,Lúcia Vieira; Alves,Milena Ferreira; Andrade,Francisco Pereira de.
Mocó cotton was the most important crop in Northeastern Brazil. Local varieties had been developed before governmental breeding programs, which should be identified and preserved as a genetic resource. The aim of this work was to select SSR markers which can be used to monitor gene flow from herbaceous to mocó cotton plants, using non transgenic upland cotton as a pollen source. Unique mocó cotton feral populations were identified in the state of Rio Grande do Norte, and mother plants and their seeds were collected. Allele frequencies were studied among fifteen plants from these populations using 64 SSR primer pairs. Ten SSR markers, with alleles exclusive to mocó or upland genotypes, were selected to monitor gene flow by paternity analysis (BNL3627; 2960;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosafety; Molecular markers; Genetic resources.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902012000100020
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Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance PAB
Pinto,Fernanda de Oliveira; Maluf,Mirian Perez; Guerreiro-Filho,Oliveiro.
The objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'-UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leucoptera coffeella; Microsatellites; Molecular markers.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000300011
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Tandem repeat distribution of gene transcripts in three plant families Genet. Mol. Biol.
Maia,Luciano Carlos da; Souza,Velci Queiróz de; Kopp,Mauricio Marini; Carvalho,Fernando Irajá Félix de; Oliveira,Antonio Costa de.
Tandem repeats (microsatellites or SSRs) are molecular markers with great potential for plant genetic studies. Modern strategies include the transfer of these markers among widely studied and orphan species. In silico analyses allow for studying distribution patterns of microsatellites and predicting which motifs would be more amenable to interspecies transfer. Transcribed sequences (Unigene) from ten species of three plant families were surveyed for the occurrence of micro and minisatellites. Transcripts from different species displayed different rates of tandem repeat occurrence, ranging from 1.47% to 11.28%. Both similar and different patterns were found within and among plant families. The results also indicate a lack of association between genome size...
Tipo: Info:eu-repo/semantics/article Palavras-chave: SSR; EST; Comparative genomics; Molecular markers.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400025
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Taxonomic and evolutionary analysis of Zaprionus indianus and its colonization of Palearctic and Neotropical regions Genet. Mol. Biol.
Commar,Leliane Silva; Galego,Luis Gustavo da Conceição; Ceron,Carlos Roberto; Carareto,Claudia Marcia Aparecida.
Zaprionus indianus is a dipteran (Drosophilidae) with a wide distribution throughout the tropics and temperate Palearctic and Nearctic regions. There have been proposals to reclassify the genus Zaprionus as a subgenus or group of the genus Drosophila because various molecular markers have indicated a close relationship between Zaprionus species and the immigrans-Hirtodrosophila radiation within Drosophila. These markers, together with alloenzymes and quantitative traits, have been used to describe the probable scenario for the expansion of Zaprionus indianus from its center of dispersal (Africa) to regions of Asia (ancient dispersal) and the Americas (recent dispersal). The introduction of Z. indianus into Brazil was first reported in 1999 and the current...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alloenzyme; Bioinvasion; Molecular markers; Phylogenetic analysis; Quantitative traits.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300003
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Terrestrial planarians (Platyhelminthes, Tricladida, Terricola) from the Iberian peninsula: first records of the family Rhynchodemidae, with the description of a new Microplana species Naturalis
Mateos, Eduardo; Giribet, Gonzalo; Carranza, Salvador.
The first records of terrestrial planarians belonging to the family Rhynchodemidae are reported for the Iberian Peninsula. A new endemic species from the Spanish Pyrenees, Microplana nana sp. nov., is described. The characteristic features of this species are: i) small size (8-10 mm) in adult individuals, ii) very long conical penis papilla and iii) absence of seminal vesicle, bursa copulatrix, genito-intestinal duct, and well-developed penial bulb. Moreover, the widespread common European land planarian Microplana terrestris (Müller, 1774) is reported for the first time from the Iberian Peninsula. The two species, M. nana sp. nov. and M. terrestris, are described by means of external morphology using histological sections, and have been characterized by...
Tipo: Article / Letter to the editor Palavras-chave: New species; ITS-1; Molecular markers; Iberian peninsula; Microplana; Rhynchodemidae; Terricola.
Ano: 1998 URL: http://www.repository.naturalis.nl/record/534286
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The exotic jumping snail Ovachlamys fulgens (Gude, 1900) (Gastropoda: Helicarionidae) in urban areas of the Upper-Paraná Atlantic Forest Anais da ABC (AABC)
BELTRAMINO,ARIEL A.; VOGLER,ROBERTO E.; RUMI,ALEJANDRA; GUZMÁN,LEILA B.; MARTÍN,STELLA M.; PESO,JUANA G..
ABSTRACT The exotic land gastropod Ovachlamys fulgens (Gude, 1900), aka the jumping snail, was registered in Eldorado city, Misiones province, constituting the first documented record of that species in Argentina-and for all of South America as well. Identities of the individuals were confirmed through morphological examination, and by a DNA sequencing of the cytochrome oxidase subunit I gene. DNA sequences for the mitochondrial 16S-rRNA and the nuclear 18S-rRNA genes were also obtained from the morphologically-confirmed specimens to gain insights into the genetic background of this species in Argentina, and as additional markers for enhancing the rapidity of identification by governmental authorities who are responsible for managing the presence of exotic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Argentina; Introduced species; Misiones province; Molecular markers; Nonnative snail.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401591
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The genetic dissection of quantitative traits in crops Electron. J. Biotechnol.
Semagn,Kassa; Bjørnstad,Åsmund; Xu,Yunbi.
Most traits of interest in plant breeding show quantitative inheritance, which complicate the breeding process since phenotypic performances only partially reflects the genetic values of individuals. The genetic variation of a quantitative trait is assumed to be controlled by the collective effects of quantitative trait loci (QTLs), epistasis (interaction between QTLs), the environment, and interaction between QTL and environment. Exploiting molecular markers in breeding involve finding a subset of markers associated with one or more QTLs that regulate the expression of complex traits. Many QTL mapping studies conducted in the last two decades identified QTLs that generally explained a significant proportion of the phenotypic variance, and therefore, gave...
Tipo: Journal article Palavras-chave: Association mapping; Linkage disequilibrium; Markers assisted selection; Molecular breeding; Molecular markers; Quantitative trait; QTL mapping; QTL analysis.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500016
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The genetic diversity of strawberry (Fragaria ananassa Duch.) hybrids based on ISSR markers - doi: 10.4025/actasciagron.v35i4.16737 Agronomy
Nunes, Claudinéia Ferreira; Empresa de Pesquisa Agropecuária de Minas Gerais; Ferreira, Juliano Lino; Empresa de Pesquisa Agropecuária de Minas Gerais; Generoso, Andressa Leal; Empresa de Pesquisa Agropecuária de Minas Gerais; Carvalho, Mário Sérgio Dias; Empresa de Pesquisa Agropecuária de Minas Gerais; Pasqual, Moacir; Universidade Federal de Lavras; Cançado, Geraldo Magela de Almeida; Empresa de Pesquisa Agropecuária de Minas Gerais.
The strawberry is an important agricultural crop in Brazil. However, most of the commercial genotypes currently in cultivation in Brazil were developed in other countries with environmental adaptations often inadequate for the regional conditions. In this work, inter-simple sequence repeat markers were used to determine the genetic variability and the loci segregation profiles of 84 strawberry hybrids obtained from a genetic breeding program at the ‘Empresa de Pesquisa Agropecuária de Minas Gerais.’ The hybrids were produced from crosses involving the following progenitors: ‘Toyonoka’ x ‘Sweet Charlie’, ‘Camino Real’ x ‘Sweet Charlie’, ‘Oso Grande’ x ‘Sweet Charlie’, ‘Oso Grande’ x ‘Toyonoka’, ‘Dover’ x ‘Oso Grande’, and ‘Camino Real’ x ‘Toyonoka’....
Tipo: Marcadores moleculares Palavras-chave: 5.01.03.05-9 genetic dissimilarity; Pre-breeding; Molecular markers Melhoramento Vegetal genetic dissimilarity; Pre-breeding; Molecular markers.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16737
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The use of microsatellite markers in Neotropical studies of wild birds: a literature review Anais da ABC (AABC)
MOURA,RENAN F.; DAWSON,DEBORAH A.; NOGUEIRA,DENISE M..
ABSTRACT Despite extensive habitat fragmentation, the Neotropical region possesses 30% of the world´s bird species. Microsatellites have remained one of the most popular genetic markers and have been used in ecological and conservation studies since the 1990’s. We conducted a literature review comparing the number of papers published from January 1990 to July 2015 that used microsatellite markers for studies of wild birds in the Neotropical region, USA and some European countries. We assigned the articles to three categories of studies: population genetics, animal behavior/kinship analysis and the development of species-specific bird microsatellite markers. We also compared the studies in the Neotropics that used heterologous versus species-specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AVES; Cross-species amplification; Biodiversity; Ecology; Molecular markers; STR.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100145
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Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level BJM
Delamare,Ana Paula Longaray; Artico,Liane de Oliveira; Grazziotin,Felipe Gobbi; Echeverrigaray,Sergio; Costa,Sérgio Olavo Pinto da.
Fifteen well-defined strains of Aeromonas of thirteen species were analyzed by SDS protein electrophoretic analysis (SDS-PAGE) and random amplified polymorphic DNA analysis (RAPD). The comparison between the patterns obtained by both methods allowed differentiating all the strains. Clusters formed by the unweighted pair group method with arithmetic averages applied to protein data correlates with the genetic and biochemical information about the species. The results show that protein fingerprinting has the potential to differentiate Aeromonas species, but the low qualitative variation indicates that this technique is not efficient for the characterization of strains within a species. Conversely, RAPD fingerprinting allows the identification of strains but...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Molecular markers; Species identification.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400016
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Transferability of heterologous microsatellite primers in Brycon gouldingi Ciência Rural
Souza,Felipe Pinheiro de; Lima,Ed Christian Suzuki de; Leite,Natalia Gonçalves; Urrea-Rojas,Angela Maria; Yamachita,Andrei Lincoln; Pandolfi,Victor César Freitas; Lopera-Barrero,Nelson Mauricio.
ABSTRACT: Brycon gouldingi is a species of neotropical fish of socioeconomic and environmental importance in the Tocantins-Araguaia Basin. Genetic studies on this species are still limited, making it difficult to evaluate the population structure and genetic diversity in natural and captive stocks. Here, we aimed to evaluate the transferability of heterologous microsatellite primers in B. gouldingi. A total of 30 primers for eight species were evaluated: Brycon hilarii, Brycon opalinus, Brycon cephalus, Brycon orbignyanus, Prochilodus lineatus, Prochilodus argenteus, Piaractus mesopotamicus, and Colossoma macropomum. The primers that showed the best amplification patterns were applied to 20 specimens of B. gouldingi, and their genetic parameters were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Cross-amplification; Molecular markers; SSR.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100406
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Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi Electron. J. Biotechnol.
Lourdes Rocha,Carla Maria; Vellicce,Gabriel Ricardo; García,María Gabriela; Pardo,Esteban Mariano; Racedo,Josefina; Perera,María Francisca; de Lucía,Adrian; Gilli,Javier; Bogado,Noelia; Bonnecarrère,Victoria; German,Silvia; Marcelino,Francismar; Ledesma,Fernando; Reznikov,Sebastián; Ploper,Leonardo Daniel; Welin,Bjorn; Castagnaro,Atilio Pedro.
Background Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of...
Tipo: Journal article Palavras-chave: Asian soybean rust; Genetic variation; Glycine max; Molecular markers.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600009
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Use of AFLPS to distinguish landraces of pejibaye (Bactris gasipaes) in brazilian Amazonia Scientia Agricola
Clement,Charles R.; Sousa,Nelcimar Reis; Rodrigues,Doriane Picanço; Astolfi-Filho,Spártaco; Moreno,Yolanda Núñez; Pascual,Vicente Torres; Rodríguez,Francisco Javier Gallego.
Although the first inhabitants of western Amazonia domesticated pejibaye (Bactris gasipaes Kunth, Palmae) or peach palm for its fruits, today it is widely planted for its heart-of-palm. Like other domesticates, pejibaye presents a complex hierarchy of landraces developed before the conquest of the Americas. The existence of three landraces (Pará, Solimões, Putumayo) was proposed along the Amazonas and Solimões Rivers, Brazil, based on morphological characteristics. There are some questions remaining about the intermediate landrace being an artifact of the morphometric analysis. AFLPs were used to evaluate the relationships among samples of these putative landraces. DNA was extracted from 99 plants representing 13 populations maintained in the Pejibaye...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular markers; Population differentiation; Genetic resources; Genetic analysis.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000400019
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Use of molecular marker techniques in seed testing by Brazilian seed companies Scientia Agricola
Della Vecchia,P.T.; Silva,C.A.R. da; Terenciano-Sobrinho,P..
Seed market is becoming global and globalization is growing very fast. To compete favourably in this new global seed world, quality and cost are and will be certanly the key issues. High seed quality can only be obtained by a thorough control of the entire seed production process, step by step from planning to final delivery. That requires science, technology, expertise, experience, good management and certanly, the most important, an absolute and unconditional commitment with quality. Seed testing for quality assurance is one important step in the process of production of high quality seed. In the late years a considerable amount of research has been published, particularly on the use of some Polymerase Chain Reaction DNA based new technologies (RAPD,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular markers; Seed testing; Seed companies; Genetic purity.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000500014
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Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases Acta Botanica
Pati,Prakash G; Datta,Subhojit; Agbagwa,Ikechukwu O; Singh,Indra Prakash; Soren,Khela Ram; Das,Alok; Choudhary,Arbind Kumar; Chaturvedi,Sushil Kumar.
Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AFLP-RGA; Cajanus cajan; Genetic diversity; Molecular markers; Polymorphism.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200007
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Variability in ribosomal DNA genic and spacer regions in Verticillium dahliae isolates from different hosts Trop. Plant Pathol.
Otero,Laura; Ducasse,Daniel; Miller,Robert N. G..
Using PCR-based assays with specific primers for amplification of the ribosomal DNA intergenic spacer region (IGS) and a portion of the mitochondrial DNA small subunit ribosomal RNA gene (mtDNA SSU rRNA), the genetic variability among Verticillium dahliae isolates from olive (Olea europaea) and other host species from Argentina and Brazil was estimated. The derived UPGMA-generated phenograms based upon the restriction fingerprinting data of rDNA IGS products revealed genetic differences, correlating with the host of origin. Isolates infecting olive genetically distinct from those from cocoa (Theobroma cacao) and sunflower (Helianthus annuus). Digestion of mitochondrial DNA SSU rRNA PCR products revealed less variability, distinguishing only one isolate...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Olea europaea; Molecular markers; Characterization.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000400015
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Variance additivity of genetic populational parameter estimates obtained through bootstrapping Scientia Agricola
Carlini-Garcia,Luciana Aparecida; Vencovsky,Roland; Coelho,Alexandre Siqueira Guedes.
Studying the genetic structure of natural populations is very important for conservation and use of the genetic variability available in nature. This research is related to genetic population structure analysis using real and simulated molecular data. To obtain variance estimates of pertinent parameters, the bootstrap resampling procedure was applied over different sampling units, namely: individuals within populations (I), populations (P), and individuals and populations simultaneously (I, P). The considered parameters were: the total fixation index (F or F IT), the fixation index within populations (f or F IS) and the divergence among populations or intrapopulation coancestry (theta or F ST). The aim of this research was to verify if the variance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Population structure; Resampling; Molecular markers; Natural populations; Simulation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000100015
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Yam: a neglected and underutilized crop in Brazil Horticultura Brasileira
Siqueira,MVBM.
In Brazil current studies and investments on yams are incipient. Similarly, the literature in recent decades lacks adequate information on this group of plants. The existing literature, on its turn, requires more than ever to be revised and organized. Yams have joined the so-called "neglected" group of crops for several reasons, but particularly because they are associated with poor and traditional communities. Many vegetables introduced in Brazil during the colonization period have adapted to different cropping systems, yams being an excellent example. This diversity resulted very widespread, yet poorly recognized in the country. In turn, the gardens using traditional farming systems continue to maintain and enhance yam local varieties. Studies from other...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dioscorea spp.; Tubers; Traditional agriculture; Agrobiodiversity; Molecular markers.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000100003
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Yield QTL analysis of Oryza sativa x O. glumaepatula introgression lines PAB
Rangel,Priscila Nascimento; Vianello,Rosana Pereira; Melo,Arthur Tavares Oliveira; Rangel,Paulo Hideo Nakano; Mendonça,João Antônio; Brondani,Claudio.
The objective of this work was to evaluate the yield performance of two generations (BC2F2 and BC2F9) of introgression lines developed from the interspecific cross between Oryza sativa and O. glumaepatula, and to identify the SSR markers associated to yield. The wild accession RS‑16 (O. glumaepatula) was used as donor parent in the backcross with the high yielding cultivar Cica‑8 (O. sativa). A set of 114 BC2F1 introgression lines was genotyped with 141 polymorphic SSR loci distributed across the whole rice genome. Molecular analysis showed that in average 22% of the O. glumaepatula genome was introgressed into BC2F1 generation. Nine BC2F9 introgression lines had a significantly higher yield than the genitor Cica‑8, thus showing a positive genome...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AB‑QTL; Breeding; Genetic pool; Molecular markers; Target genome region; Yield performance.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000300006
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การจัดทำลายพิมพ์เอกลักษณ์ดีเอ็นเอข้าวพื้นเมืองไทยที่เก็บอยู่ในธนาคารเชื้อพันธุ์ข้าวแห่งชาติ Thai Agricultural
Payorm Cobelli; Varapong Chamarerk; Poonsak Mekwatanakarn; Phikul Leelagud; Somsong Chotechuen; Sommai Seewisut.
SSR molecular markers SSR are tandem arranged repeats of short DNA motifs (1-6 bp in length) distributed throughout the genome that frequently exhibit variation in the number of repeats at the locus of each rice variety. The SSR markers, therefore, are suitable for detecting polymorphisms between closely related genotypes of Thai local rice varieties from the National Rice Germplasm Bank. In this research, DNA fingerprinting was conducted using 8 Thai local rice varieties. i.e., Niaw Sanpahtawng (NSPT_GB), Khao Gaw Diaw (KGD_GB), Niaw Dam (ND_GB), Khao tah Haeng 17 (KTH17_GB), Jao’ Haw (JH_GB), Pin Gaew 56 (PG56_GB), Jek Chuey Gahb Khiaw (JCGK_GB), Sang Yod Pattalung (SYPL_GB), and 5 standard varieties, i.e., Khao Dawk Mali 105 (KDML105_GB, KDML105_C),...
Tipo: PhysicalObject Palavras-chave: Thai local rice; DNA fingerprinting; Molecular markers; National Rice Germplasm Bank; ข้าวพื้นเมืองไทย; ลายพิมพ์เอกลักษณ์ดีเอ็นเอ; เครื่องหมายโมเลกุล; การเรียงตัวของดีเอ็นเอ; ความใกล้ชิดทางพันธุกรรม; พันธุ์.
Ano: 2012 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5225
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